SARS-CoV-2 (Alpha) S Protein RBD (Arg319-Phe541), with C-hFc Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP1018-50ug

  • Size

    50 ug

  • Price

    $543

1
  • SKU

    DMP1018-100ug

  • Size

    100 ug

  • Price

    $805

1
This recombinant protein contains Arg319-Phe541 of Uniprot ID P0DTC2 with one N501Y mutation. It has a C-hFc Tag, with a calculated molecular weight of 51.3 kDa. In SDS-PAGE, the protein migrates at 55-70 kDa due to glycosylation.
gene name SARS-CoV-2 S Protein RBD
alias SARS-CoV-2 B.1.1.7 (Alpha) Spike RBD Protein
host species SARS-CoV-2
production host HEK293
tags C-hFc Tag
purity The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 2 ug/mL (100 uL/well) SARS-CoV-2 (Alpha) S protein RBD, hFc Tag binds to Human ACE2 Protein, mFc Tag in a linear range of 4.115?1000 ng/mL.

ELISA plate coated with 2 ug/mL (100 uL/well) SARS-CoV-2 (Alpha) S protein RBD, hFc Tag binds to anti-SARS-CoV-2 (CB6 biosimilar) mAb in a linear range of 1.00?97.66 ng/mL.

ELISA plate coated with 2 ug/mL (100 uL/well) SARS-CoV-2 (Alpha) S protein RBD, hFc Tag binds to anti-SARS-CoV-2 RBD antibody (DM55), Rabbit mAb the antibody in a linear range of 0.244?15.625 ng/mL.

SARS-CoV-2 (2019-nCoV) S protein RBD (N501Y), hFc Tag, SDS-PAGE analysis under reducing conditions.


PRODUCT

  • SKU

    DMP1018-50ug

  • Size

    50 ug

  • Price

    $543

1
  • SKU

    DMP1018-100ug

  • Size

    100 ug

  • Price

    $805

1