SARS-CoV-2 S protein RBD (Arg319-Phe541), with C-mFc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1993-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCD1993-1mg

  • Size

    1mg

  • Price

    $3,810

1
This recombinant protein contains Arg319-Phe541 of NCBI Accession# QHD43416.1. It has a C-mFc tag and was expressed from HEK293. The protein's calculated molecular weight is 52.0 kDa, but migrates at 60-65 kDa under reducing conditions in SDS-PAGE due to glycosylation.
host species SARS-CoV-2
production host HEK293
tags C-mFc
purity >95% as determined by SDS-PAGE.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation The protein was lyophilized from a solution of PBS,pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of SARS-CoV-2 S RBD protein

ELISA: Immobilized Human ACE2-His at 1 ug/mL (100 uL/well) binds to SARS-CoV-2 S protein RBD-mFc with a linear range of 0.1-2 ng/mL.


PRODUCT

  • SKU

    YCD1993-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCD1993-1mg

  • Size

    1mg

  • Price

    $3,810

1