SARS-CoV-2 S1 protein (Val16-Arg685), with C-Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8625-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8625-1mg

  • Size

    1mg

  • Price

    $3,810

1
  • SKU

    YCP8625-1g

  • Size

    1g

  • Price

    $693,000

1
This recombinant protein contains Val16-Arg685 of NCBI Accession# QHD43416.1. It has a C-Fc tag and was expressed from HEK293. The protein's calculated molecular weight is 101.5 kDa, but migrates at 130-150 kDa under reducing conditions in SDS-PAGE due to glycosylation.
host species SARS-CoV-2
production host HEK293
tags C-Fc
purity >95% as determined by SDS-PAGE.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation The protein was lyophilized from a solution of Tris, Glycine and NaCl, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of SARS-CoV-2 S1 protein

ELISA: Immobilized Biotinylated Human ACE2-His-Avi at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate,binds to SARS-CoV-2 S1 protein-Fc with a linear range of 0.4-6 ng/mL.

ELISA: Immobilized SARS-CoV-2 S1 protein-Fc at 1 ug/mL (100 uL/well) binds to Anti-SARS-CoV-2 Neutralizing Antibody with a linear range of 0.4-6 ng/mL .


PRODUCT

  • SKU

    YCP8625-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8625-1mg

  • Size

    1mg

  • Price

    $3,810

1
  • SKU

    YCP8625-1g

  • Size

    1g

  • Price

    $693,000

1