Canine CD3E (Gln22-Leu122) and CD3D (Phe22-Thr103) heterodimer protein, with C-10xHis on CD3E, expressed from HEK293

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PRODUCT

  • SKU

    YCD1231-100ug

  • Size

    100ug

  • Price

    $570

1
  • SKU

    YCD1231-1mg

  • Size

    1mg

  • Price

    $3,900

1
This recombinant heterodimer protein contains Gln22-Leu122 (CD3E) of UniProt P27597 and Phe22-Thr103 (CD3D) of NCBI A0A8C0PNZ1. Calculated molecular weights for each chain are 16.6 kDa (CD3E) and 13.3 kDa (CD3D). Due to glycosylation, the heterodimer protein migrates at 19-20 kDa (CD3E) under reducing conditions and at 36-45 kDa (CD3D) under non-reducing conditions in SDS-PAGE. Subunit CD3E is fused with a His tag at the C-terminus and subunit CD3D is tag free.
gene name CD3E, CD3D
gene id UniProt P27597 and NCBI A0A8C0PNZ1
host species Canine
production host HEK293
tags C-10xHis & Tag-free
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Canine CD3E & CD3D Heterodimer protein


PRODUCT

  • SKU

    YCD1231-100ug

  • Size

    100ug

  • Price

    $570

1
  • SKU

    YCD1231-1mg

  • Size

    1mg

  • Price

    $3,900

1