Human CD3E / CD3 epsilon (Asp23-Asp126) protein, with C-10xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP1525-100ug

  • Size

    100ug

  • Price

    $577.5

1
  • SKU

    YCP1525-1mg

  • Size

    1mg

  • Price

    $3,960

1
This recombinant protein contains Asp23-Asp126 of UniProt P07766. It has a His tag at the C-terminus. The protein's calculated molecular weight is 16.9 kDa. Maybe caused by unknown PTMs, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).
gene name CD3E
gene id UniProt P07766
host species Human
production host HEK293
tags C-10xHis
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human CD3 epsilon protein

ELISA: Immobilized Human CD3 epsilon-His at 1 ug/mL (100 uL/well) binds to Mouse Anti-Human CD3 (SP34-2) with a linear range of 0.2-3 ng/mL.

ELISA: Immobilized Mouse Anti Human CD3(SP-34) at 2 ug/mL (100 uL/well) binds to Human CD3 epsilon-His with a linear range of 0.2-2 ng/mL .


PRODUCT

  • SKU

    YCP1525-100ug

  • Size

    100ug

  • Price

    $577.5

1
  • SKU

    YCP1525-1mg

  • Size

    1mg

  • Price

    $3,960

1