Human CD3E (Asp23-Asp126) and CD3G (Gln23-Ser116) heterodimer protein, with C-Fc-6xHis & C-Fc-Flag Tags respectively, expressed from HEK293

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PRODUCT

  • SKU

    YCP6021-50ug

  • Size

    50ug

  • Price

    $525

1
  • SKU

    YCP6021-1mg

  • Size

    1mg

  • Price

    $4,620

1
This recombinant heterodimer protein contains Asp23-Asp126 (CD3E) of UniProt P07766 and Gln23-Ser116 (CD3G) of UniProt P09693. Each chain has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G) but migrates at 40-50 kDa due to glycosylation. Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a His tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag.
gene name CD3E, CD3G
gene id UniProt P07766 (CD3E), P09693 (CD3G)
host species Human
production host HEK293
tags C-Fc-6xHis & C-Fc-Flag
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human CD3E&CD3G Heterodimer protein

ELISA: Immobilized Human CD3E&CD3G Heterodimer Protein at 2 ug/mL (100 uL/well) binds to UCHT1 with a linear range of 0.4-6 ng/mL.

ELISA: Immobilized Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag at 1 ug/mL (100 uL/well) binds to Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with a linear range of 0.4-13 ng/mL .


PRODUCT

  • SKU

    YCP6021-50ug

  • Size

    50ug

  • Price

    $525

1
  • SKU

    YCP6021-1mg

  • Size

    1mg

  • Price

    $4,620

1