Human CD3E (Asp23-Asp126) and CD3D (Phe22-Ala105) heterodimer protein, with C-Fc-10xHis and C-Fc-Flag Tag respectively, expressed from HEK293

Home/ Products / ECD and Other Proteins

PRODUCT

  • SKU

    YCD1250-100ug

  • Size

    100ug

  • Price

    $1,050

1
  • SKU

    YCD1250-1mg

  • Size

    1mg

  • Price

    $3,810

1
This recombinant protein contains Asp23-Asp126 (CD3E) of UniProt P07766 and Phe22-Ala105(CD3D) of UniProt P04234. The heterodimer protein was produced by co-expression of CD3E and CD3D. The proteins calculated molecular weights are 40.2 kDa (CD3E) and 37.3 kDa (CD3D) for each chain, but migrate at 45-60 kDa under reducing conditions and 100-116 kDa under non-reducing conditions in SDS-PAGE due to glycosylation. Subunit CD3E is fused with a human IgG1 Fc tag and a His tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus.
gene name CD3E, CD3D
gene id UniProt P07766 (CD3E), P04234 (CD3D)
host species Human
production host HEK293
tags C-Fc-10xHis & C-Fc-Flag
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human CD3E&CD3D Heterodimer protein

ELISA: Immobilized Human CD3E&CD3D Heterodimer Protein at 1 ug/mL (100 uL/well) binds to Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with a linear range of 0.2-3 ng/mL.

ELISA: Immobilized Human CD3E&CD3D Heterodimer Protein at 1 ug/mL (100 uL/well) binds to UCHT1 with a linear range of 0.1-3 ng/mL .


PRODUCT

  • SKU

    YCD1250-100ug

  • Size

    100ug

  • Price

    $1,050

1
  • SKU

    YCD1250-1mg

  • Size

    1mg

  • Price

    $3,810

1