Human CD3E (Asp23-Asp126) and CD3D (Phe22-Ala105) heterodimer protein, with C-Fc-10xHis and C-Fc-Flag Tag respectively, expressed from HEK293

This recombinant protein contains Asp23-Asp126 (CD3E) of UniProt P07766 and Phe22-Ala105(CD3D) of UniProt P04234. The heterodimer protein was produced by co-expression of CD3E and CD3D. The proteins calculated molecular weights are 40.2 kDa (CD3E) and 37.3 kDa (CD3D) for each chain, but migrate at 45-60 kDa under reducing conditions and 100-116 kDa under non-reducing conditions in SDS-PAGE due to glycosylation. Subunit CD3E is fused with a human IgG1 Fc tag and a His tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus.